Name: 
 

Biol 2421-Test 2B Review



Multiple Choice
Identify the letter of the choice that best completes the statement or answers the question.
 

1. 

Even though bacteria can be distinguished with the microscope, differentiation is limited.  However, bacteria have evolved diverse enzymes enabling to them to obtain __ and adapt to various environmental conditions.  Thus, bacteria in __ culture may be differentiated according to size, shape, color, culture characteristics and metabolic pattern of digestive enzymes.
a.
space, mixed
b.
nutrients, pure
 

2. 

In embarking upon a microbial study, a source is sampled.  What ought the investigator consider?
a.
what sample to take
b.
why take that sample
c.
method to obtain the sample
d.
how to process sample
e.
all of these
 

3. 

In order to perceive a microbe macroscopically, a cell must first be:
a.
colored
b.
streaked
c.
multiplied
d.
enlarged
e.
sterilized
 

4. 

Microbial media is used for:
a.
primary isolation,
b.
maintain cultures
c.
bacterial identification
d.
transport
e.
all these
 

5. 

A fastidious organism:
a.
is a fast grower
b.
fasts before growing
c.
is a finicky feeder
d.
exhibits quick motility
e.
is acid-fast
 

6. 

The pH indicator dye/s incorporated in selective and differential media:
a.
phenol red
b.
crystal violet
c.
neutral red
d.
methylene blue
e.
all of these
 

7. 

What physical mode of microbial control relies on mechanical separation of microbes from some fluid medium?
a.
heat
b.
radiation
c.
ultrasonication
d.
filtration
e.
pasteurization
 

8. 

Methods typically employed to sterilize or __ objects are too harsh for safe application to live tissue.  A milder treatment, for skin cleansing, for example, employs __ to achieve microbial control.
a.
disinfect, antiseptics
b.
disintegrate, germicides
 

9. 

Walled bacteria are shielded from __ lysis; any agent that weaken or prevents the synthesis of __ jeopardizes survival.
a.
hypertonic, cellulose
b.
hypotonic , peptidoglycan
 

10. 

In order to kill bacterial endospores at 121° C, dry heat requires about __ times longer exposure than moist heat.
a.
2
b.
5
c.
100
d.
3
e.
40
 

11. 

Pasteurization is also used to disinfect beer, wine, fruit juices or __ during preparation.  Milk can be sterilized by __ temperature of 134°C for 1-2 seconds.
a.
blood, dry
b.
beverages, ultrahigh
 

12. 

Boiling water before drinking is a practical precaution after a flood or some disruption that jeopardizes drinking water supplies.  Susceptible enterics, the typhoid and __ bacilli which do not form __ or cysts, can thus be killed.
a.
cholera, spores
b.
Giardia, trophozoites
 

13. 

The most potent form of radiant energy for microbial control is/are:
a.
gamma radiation
b.
ultraviolet radiation
c.
infrared rays
d.
radio waves
e.
colored (visible) light
 

14. 

All of the following are halogens except:
a.
methionine
b.
chlorine
c.
fluorine
d.
iodine
e.
bromine
 

15. 

Phenol, an __ compound also called phenolic acid, is an extract of coal tar, a product of petroleum distillation.  Though __ and poisonous, phenol gained historical significance by the pioneering efforts of __ in surgical antisepsis.  It remains one of the standards in the evaluation of certain disinfectants.
a.
obsolete, carcinogenic, Koch
b.
aromatic, acrid, Lister
 

16. 

Hydrogen peroxide, H2O2, is an unstable __ that decomposes readily in __ and metallic contact.  It is used in the __ test to differentiate bacteria, breaking down into H2O and O2 bubbles.
a.
oxidant, light, catalase
b.
halogen, air, aerobic
 

17. 

Bacterial death will result from damage to which of the following structures?
a.
cell wall
b.
plasma membrane
c.
proteins
d.
nucleic acids
e.
all of the above
 

18. 

In redox reactions, the substrate that loses electrons is __ and the substrate that accepts electrons is __.
a.
reduced, oxidized
b.
oxidized, reduced
 

19. 

Which of the following compounds has the greatest amount of POTENTIAL energy?
a.
CO2
b.
ATP
c.
Glucose
d.
ADP
e.
Steric acid (a fatty acid)
 

20. 

A compound that is being REDUCED in a chemical reaction is the one that:
a.
has lost hydrogen.
b.
has gained hydrogen.
c.
has gained oxygen.
d.
has gained phosphate.
e.
has lost phosphate.
 

21. 

Although enzymes are reusable, eventually they wear out and lose function.  Hence chronic __ deficiency results in the depletion of __ and downstream metabolism suffers unless an alternative pathway compensates.
a.
cofactor, coenzymes
b.
coenzyme, cofactors
c.
metal, substrates
d.
vitamin, coenzymes
e.
all of these
 

22. 

Which of the following is not true of NAD or nicotinamide adenine dinucleotide?
a.
is an apoenzyme component
b.
deletes electrons, protons
c.
final electron acceptor
d.
undergoes redox reactions
e.
is from niacin, nicotinic acid
 

23. 

Phosphate groups manifest all these properties except:
a.
PO4- is a large entity
b.
one PO4- group repels the other
c.
fructose di-PO4- yields or splits under strain
d.
PO4- highly electropositive
e.
strains from bulk & repulsion
 

24. 

Each NADH + H+ can generate ____________ ATP during oxidative phosphorylation:
a.
1
b.
3
c.
6
d.
12
e.
38
 

25. 

The net equation for aerobic respiration is:
a.
C6H12O6 + 602 ® 6CO2 + 6H2O
c.
C6H12O6 ® 2C2H5OH + 2CO2
b.
6CO2 + 6H2O ® C6H12O6 + 602
d.
2C2H5OH + 2CO2 ® C6H12O6
 



 
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