Multiple Choice
Identify the letter of the choice that
best completes the statement or answers the question.
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1.
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Even
though bacteria can be distinguished with the microscope, differentiation is limited. However,
bacteria have evolved diverse enzymes enabling to them to obtain __ and adapt to various
environmental conditions. Thus, bacteria in __ culture may be differentiated according to size,
shape, color, culture characteristics and metabolic pattern of digestive enzymes. a. | space, mixed | b. | nutrients, pure | | | | |
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2.
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The
ideal prerequisite for microbial detection and study is to manipulate microbes so they can
be: a. | seen | b. | heard | c. | smelled | d. | tasted | e. | felt | | |
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3.
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Typical clinical or environmental specimens consist of __ microbial populations and
the one of interest may be __ in the minority. a. | mixed, hidden | b. | pure, mixed | | | | |
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4.
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The
deliberate introduction of a small sample of a microbial specimen into transport-, growth-, or
storage media constitutes an inoculum whereas unintentional intrusion is not. Thus we distinguish
between the __ versus the __. a. | inoculation, contamination | b. | contamination, inoculation | | | | |
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5.
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In
embarking upon a microbial study, a source is sampled. What ought the investigator
consider? a. | what sample to
take | b. | why take that
sample | c. | method to obtain
the sample | d. | how to process
sample | e. | all of
these | | |
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6.
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The
cultivated growth following inoculation of any nutrient media is called a:
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7.
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A
colony on or embedded in solid media is pure if it is not touching a neighbor. a. | always true | b. | usually true | | | | |
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8.
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The
streak plate method is __ economical than the loop dilution method in terms of time and materials
since streaking can achieve isolation in __ incubation step. a. | less, half an | b. | more, one | | | | |
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9.
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Typical clinical specimens for diagnosis include all but: a. | blood, cerebrospinal fluid | b. | sputum, tears | c. | open lesion exudate | d. | urine, feces | e. | soil, water | | |
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10.
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In
order to perceive a microbe macroscopically, a cell must first be: a. | colored | b. | streaked | c. | multiplied | d. | enlarged | e. | sterilized | | |
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11.
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Broths, milks, or infusions are generally __ media that remain __ at refrigerator
temperature. a. | suspension,
settled | b. | liquid, fluid | | | | |
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12.
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The
following items apply to liquid nutrient media except: a. | watery in refrigerator | b. | includes litmus milk | c. | spill proof | d. | broths | e. | infusions | | |
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13.
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In SIM
medium, what does the I stands for? a. | iron sulfide | b. | indole reaction | | | | |
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14.
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In SIM
medium iron sulfide manifests by a change of a. | blue to colorless | b. | pink to red | c. | liquid to solid | d. | original to black | e. | agar to gelatin | | |
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15.
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The
basic distinction between a nutrient broth and solid media is in the absence or presence of
a: a. | dissolving
agent | b. | solidifying agent | | | | |
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16.
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Like
MacConkey agar, __ broth and __ agar are rich in nutrients suitable for hard-to-grow
microbes. a. | gelatin,
minimal | b. | nutrient, blood | | | | |
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17.
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Nutrient medium containing blood, serum, vitamins and growth factors needed for
fastidious organisms is characteristic of: a. | general purpose media | b. | enriched media | | | | |
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18.
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A
fastidious organism: a. | is a fast grower | b. | fasts before growing | c. | is a finicky feeder | d. | exhibits quick motility | e. | is acid-fast | | |
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19.
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Staphylococcus aureus is adapted to mildly salty environments like the skin and
nares. Mannitol salt agar (7.5% NaCl) will __ S. aureus growth but not accompanying bacteria
__ to that concentration of salt. a. | permit, sensitive | b. | suppress, tolerant | | | | |
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20.
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In
general, gram __ bacteria are inhibited by bile salts more than gram __ enteric
bacteria. a. | positive,
negative | b. | negative, positive | | | | |
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21.
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Differential media are also selective media if they contain
discriminating:
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22.
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The pH
indicator dye/s incorporated in selective and differential media: a. | phenol red | b. | crystal violet | c. | neutral red | d. | methylene blue | e. | all of these | | |
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23.
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The
chief purpose of incubation is to: a. | allow bacteria to self-destruct | b. | isolate pathogens | c. | arrest fermentation | d. | promote multiplication | e. | stain subcultures | | |
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24.
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The
preparation of a secondary (or subsequent) culture from the original is called: a. | isolation | b. | subculture | c. | inspection/identification | d. | incubation | e. | inoculation | | |
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25.
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Unneeded cultures are best autoclaved or arrested by: a. | refrigeration | b. | incubation | c. | inoculation | d. | incineration | e. | isolation | | |
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