Multiple Choice
Identify the
letter of the choice that best completes the statement or answers the question.
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1.
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Place
the steps of the Gram stain in the correct order:
1-Alcohol-acetone
2-Crystal violet
3-Safranin
4-Iodine. a. | 1-2-3-4 | b. | 2-1-4-3 | c. | 2-4-1-3 | d. | 4-3-2-1 | e. | 1-3-2-4 | | |
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2.
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Which
of the following pairs is mismatched? a. | Alcohol-acetone--decolorizer | b. | Crystal violet--basic
dye | c. | Safranin--acid
dye | d. | Iodine--mordant | e. | None of these | | |
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3.
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The chief purpose
of incubation is to: a. | allow bacteria to self-destruct | b. | isolate pathogens | c. | arrest fermentation | d. | promote multiplication | e. | stain subcultures | | |
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4.
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The procedure that
kills cells and binds them to the glass slide is called (a): a. | smear preparation | b. | heat fixing | c. | hanging drop | d. | staining | e. | wet mount | | |
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5.
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In which class of
stain is the color-bearing ion negatively charged? a. | basic (cationic) dyes | b. | acidic (anionic) dyes | | | | |
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6.
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Bacterial cells
are generally __ charged and thus attract __ dyes. a. | negatively, basic | b. | positively, acidic | c. | positively, basic | d. | negatively, acidic | e. | not, neither | | |
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7.
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Microorganisms are suitable laboratory subjects of study because of they
are: a. | microscopic | b. | fast growing | c. | highly adaptable | d. | comparatively
simple | e. | all of these | | |
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8.
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It means barren or
entirely devoid of life. a. | sterile | c. | spoilage | b. | infectious | d. | decay | | | | |
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9.
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The
purpose of applying refractive (immersion) oil to bridge the gap between the objective and slide is
NOT to: a. | displace
air | b. | optimize
resolution | c. | capture peripheral light | d. | hold the slide down | e. | effectively raise numerical
aperture | | |
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10.
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This
is not a typical differential stain: a. | capsule stain | b. | endospore stain | c. | simple stain | d. | Gram stain | | |
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