Name: 
 

Methods and Metabolism Review (Chps. 3, 7, & 8)



Multiple Choice
Identify the letter of the choice that best completes the statement or answers the question.
 

1. 

Pick the indispensible laboratory substance extracted from seaweed, containing galactose and sulfur:
a.
agar
b.
peptidoglycan
c.
glycocalyx
d.
chitin
e.
cellulose
 

2. 

Even though bacteria can be distinguished with the microscope, differentiation is limited.  However, bacteria have evolved diverse enzymes enabling to them to obtain __ and adapt to various environmental conditions.  Thus, bacteria in __ culture may be differentiated according to size, shape, color, culture characteristics and metabolic pattern of digestive enzymes.
a.
space, mixed
b.
nutrients, pure
 

3. 

The ideal prerequisite for microbial detection and study is to manipulate microbes so they can be:
a.
seen
b.
heard
c.
smelled
d.
tasted
e.
felt
 

4. 

Typical clinical or environmental specimens consist of __ microbial populations and the one of interest may be __ in the minority.
a.
mixed, hidden
b.
pure, mixed
 

5. 

Broths, milks, or infusions are generally __ media that remain __ at refrigerator temperature.
a.
suspension, settled
b.
liquid, fluid
 

6. 

The basic distinction between a nutrient broth and solid media is in the absence or presence of a:
a.
dissolving agent
b.
solidifying agent
 

7. 

Microbial media is used for:
a.
primary isolation,
b.
maintain cultures
c.
bacterial identification
d.
transport
e.
all these
 

8. 

Nutrient medium containing blood, serum, vitamins and growth factors needed for fastidious organisms is characteristic of:
a.
general purpose media
b.
enriched media
 

9. 

Staphylococcus aureus is adapted to mildly salty environments like the skin and nares. Mannitol salt agar (7.5% NaCl) will __ S. aureus growth but not accompanying bacteria __ to that concentration of salt.
a.
permit, sensitive
b.
suppress, tolerant
 

10. 

In general, gram __ bacteria are inhibited by bile salts more than gram __ enteric bacteria.
a.
positive, negative
b.
negative, positive
 

11. 

The pH indicator dye/s incorporated in selective and differential media:
a.
phenol red
b.
crystal violet
c.
neutral red
d.
methylene blue
e.
all of these
 

12. 

The preparation of a secondary (or subsequent) culture from the original is called:
a.
isolation
b.
subculture
c.
inspection/identification
d.
incubation
e.
inoculation
 

13. 

The biological forms most resistant to microbial control are (the):
a.
vegetative cells
b.
bacterial, fungal spores
c.
enveloped viruses
d.
trophozoites, cysts
e.
gram positive bacteria
 

14. 

Boiling water or steam are forms of __ heat that are more efficient antimicrobically than heated dry air, except perhaps, for __.
a.
moist, incineration
b.
chemical, exothermal reaction
 

15. 

Typical laboratory membrane filtration employs a filter of known pore __ that is placed in a graduated funnel attached to a vacuum flask.  Filters with pores smaller than viruses yield __ fluids.
a.
number, antiseptic
b.
size, sterile
 

16. 

Which of the following could be used to sterilize plastic Petri plates in a plastic wrapper?
a.
ultraviolet
b.
ionizing radiation
c.
visible light
d.
dry heat
e.
pasteurization
 

17. 

Bacterial death will result from damage to which of the following structures?
a.
cell wall
b.
plasma membrane
c.
proteins
d.
nucleic acids
e.
all of the above
 

18. 

Which concentration of ethyl or isopropyl alcohol is the most effective bactericide?
a.
100%
b.
70%
c.
50%
d.
25%
e.
10%
 

19. 

A compound that is being REDUCED in a chemical reaction is the one that:
a.
has lost hydrogen.
b.
has gained hydrogen.
c.
has gained oxygen.
d.
has gained phosphate.
e.
has lost phosphate.
 

20. 

What is the fate of pyruvic acid (pyruvate) in an organism that uses aerobic respiration?
a.
It is reduced to lactic acid.
b.
It is further oxidized in the Krebs cycle.
c.
It is oxidized in the electron transport chain.
d.
It is catalysed in glycolysis.
e.
It is reduced in the Krebs cycle.
 

21. 

The end products of aerobic cellular respiration are ATP energy, :
a.
O2 and H2O.
b.
CO2 and H2O.
c.
pyruvate and glucose.
d.
CO2 and O2.
e.
pyruvate and CO2
 

22. 

E. coli is a facultatively anaerobic organism.  The fermentative end product(s) of this organism is/are:
a.
mixed acids
b.
CO2 and H2O
c.
lactic acid
d.
ethanol and carbon dioxide
e.
butanediol and acetoin
 

23. 

Enzymes, organic catalysts, are reused since they:
a.
increase reactivity
b.
bind reactants
c.
are released intact
d.
facilitate certain reactions
e.
all of these
 

24. 

_____is not an electron carrier associated with the respiratory chain.
a.
pyruvic acid
b.
NADP
c.
NADH
d.
coenzyme A
e.
FAD
 

25. 

An environment that furnishes only inorganic salts and organic compounds as final electron acceptors, cannot support __ organisms.
a.
facultative anaerobes
b.
aerotolerant
c.
aerobic
d.
strict anaerobes
e.
all of these
 



 
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